Spin ikholamu

Spin ikholamu

Tsananguro Pfupi:

Iyo spin yekoramu yakazadzwa nehombe yakawanda yegirazi fiber membrane, inoenderana negirazi fiber yekubvisa nzira yekukurumidza nucleic acid yekubvisa tekinoroji. Makoramu ekuchenesa anogona kushandiswa kuchenesa imwechete - kana yakapetwa kaviri DNA kana RNA kubva kune tsvina ine munyu, solvents, enzyme, kana mapuroteni. Iyo nucleic acid inoshambadzirwa kune iyo girazi faibha pane yakanyanya munyu musungwa, uye munyu uye marara zvinobviswa mune yekuchenesa nhanho. Yakachena DNA / RNA inoshambidzwa ichibviswa nemvura nemvura kana TE buffer. Nehunhu hwekushanda kuri nyore, kudzoreredza mwero uye kuita kwakadzikama, chigadzirwa chakashandiswa nemarabhoritari mazhinji nemakambani epamba nekunze. Iyo nucleic acid yekuchenesa ikholamu inoshandisa silica gel membrane seyakanyanya adsorption zvinhu zveiyo nucleic acid, nepo kushambadzira kwezvimwe zvipenyu zvinhu zvisizvo kwete, izvo zvinogona kuve nechokwadi chekukwirisa kudzoreredzwa kweDNARNA mumuenzaniso, uku uchibvisa zvimwe zvisina kuchena.


Chigadzirwa Chigadzirwa

Chigadzirwa Mateki

Unhu hwechigadzirwa

DNA / RNA micropurification ikholamu

Inoenderana neQIAGEN uye Invitrogen buffers

Iko kusungira kugona kwaive 45 ~ 50 g

Kukura kwechimedu kwakatangira kubva pa65bp kusvika pa10kbp

Iyo buffer fomula inokodzera plasmid diki-gadziriro yekugadzirira, gel yekubvisa uye PCR clearance

Chigadzirwa chinoshandiswa

Kukurumidza kuchenesa kwePCR zvigadzirwa zvekusimudzira

Kudzoreredza kweDNA tambo kubva kuagarose gel

Kubviswa kwePlasmid DNA

Genomic DNA Kubvisa

RNA kuchenesa

Isolate yeiyo chaiyo DNA mune yekuita musanganiswa

Chigadzirwa maficha

Nyore oparesheni uye yakakwirira yekuwedzera kugona.

Yakakwirira yekubvisa kugona.

Iyo yakaburitswa genomic DNA / RNA ine kutendeka kwakanaka uye kwakanyanya kuchena.

Yakakwira mhando silica gel firimu, yakanaka DNA / RNA kushambadzira kuita.

Inesarudzo 4.6.8.12 dura

Iyo yekuunganidza chubhu yenucleic acid yekuchenesa ikholamu inogadzirwa neyekurapa giredhi polypropylene

Iyo Column yeiyo nucleic acid yekuchenesa ikholamu inogadzirwa neyekurapa giredhi polypropylene ine mesh kana interface pazasi

Iyo gasket yeiyo nucleic acid yekuchenesa ikholamu yakasarudzika yakasviba zvinhu, iyo inopesana neasidhi uye hwaro uye akawanda manyoro manyoro, uye haishambadze akawanda ehupenyu mamorekuru.

Iyo yakanyatsogadziriswa silica gel firimu kana girazi faibha firimu yezvinhu zvakaunzwa kunze zvigadzirwa zvinogona kushambadza nucleic acid mamorekuru kusvika pakukwirira kusvika.

AIHWA. Tsananguro Filter akaturikidzana Ruvara rwemhete Sefa Cap Vhoriyamu Kurongedza / Ctns
PC0001 Spin Column / / / 2ml Bulk 10000
Bhegi 8000
PC0008 Spin Column 2 Bhuruu Magirazi egirazi 0.8ml Bulk 12000
Bhegi 10000
PC0009 Spin Column 4 tsvuku Magirazi egirazi 0.8ml Bulk 12000
Bhegi 10000
PC0010 Spin Column 1 / PTFE firita 0.8ml Bulk 12000
Bhegi 10000
PC0019 96 tsime rekubvisa ndiro 6 Bhuruu Silica nhengo × 96 * 1.0ml Bhegi 50
PC0027 Spin Column 8 Pepuru Silica nhengo 0.8ml Bulk 12000
Bhegi 10000
PC0028 Spin Column 4 Transparent Silica nhengo 0.8ml Bulk 12000
Bhegi 10000
PC0033 Spin Column 6 tsvuku Silica nhengo 0.8ml Bulk 12000
Bhegi 10000
PC0054 Spin Column 12 Chiedza chitsvuku Silica nhengo 0.8ml Bulk 12000
Bhegi 10000
PC0091 O-Mhete / / NC x / Bhegi 5000

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