Spin ikholamu

Spin ikholamu

Tsananguro Pfupi:

Iyo spin yekoramu yakazadzwa nehombe yakawanda yegirazi fiber membrane, inoenderana negirazi fiber yekubvisa nzira yekukurumidza nucleic acid yekubvisa tekinoroji. Makoramu ekuchenesa anogona kushandiswa kuchenesa imwechete - kana yakapetwa kaviri DNA kana RNA kubva kune tsvina ine munyu, solvents, enzyme, kana mapuroteni. Iyo nucleic acid inoshambadzirwa kune iyo girazi faibha pane yakanyanya munyu musungwa, uye munyu uye marara zvinobviswa mune yekuchenesa nhanho. Yakachena DNA / RNA inoshambidzwa ichibviswa nemvura nemvura kana TE buffer. Nehunhu hwekushanda kuri nyore, kudzoreredza mwero uye kuita kwakadzikama, chigadzirwa chakashandiswa nemarabhoritari mazhinji nemakambani epamba nekunze. Iyo nucleic acid yekuchenesa ikholamu inoshandisa silica gel membrane seyakanyanya adsorption zvinhu zveiyo nucleic acid, nepo kushambadzira kwezvimwe zvipenyu zvinhu zvisizvo kwete, izvo zvinogona kuve nechokwadi chekukwirisa kudzoreredzwa kweDNARNA mumuenzaniso, uku uchibvisa zvimwe zvisina kuchena.

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Chigadzirwa Mateki

Unhu hwechigadzirwa

DNA / RNA micropurification ikholamu

Inoenderana neQIAGEN uye Invitrogen buffers

Iko kusungira kugona kwaive 45 ~ 50 g

Kukura kwechimedu kwakatangira kubva pa65bp kusvika pa10kbp

Iyo buffer fomula inokodzera plasmid diki-gadziriro yekugadzirira, gel yekubvisa uye PCR clearance

Chigadzirwa chinoshandiswa

Kukurumidza kuchenesa kwePCR zvigadzirwa zvekusimudzira

Kudzoreredza kweDNA tambo kubva kuagarose gel

Kubviswa kwePlasmid DNA

Genomic DNA Kubvisa

RNA kuchenesa

Isolate yeiyo chaiyo DNA mune yekuita musanganiswa

Chigadzirwa maficha

Nyore oparesheni uye yakakwirira yekuwedzera kugona.

Yakakwirira yekubvisa kugona.

Iyo yakaburitswa genomic DNA / RNA ine kutendeka kwakanaka uye kwakanyanya kuchena.

Yakakwira mhando silica gel firimu, yakanaka DNA / RNA kushambadzira kuita.

Inesarudzo 4.6.8.12 dura

Iyo yekuunganidza chubhu yenucleic acid yekuchenesa ikholamu inogadzirwa neyekurapa giredhi polypropylene

Iyo Column yeiyo nucleic acid yekuchenesa ikholamu inogadzirwa neyekurapa giredhi polypropylene ine mesh kana interface pazasi

Iyo gasket yeiyo nucleic acid yekuchenesa ikholamu yakasarudzika yakasviba zvinhu, iyo inopesana neasidhi uye hwaro uye akawanda manyoro manyoro, uye haishambadze akawanda ehupenyu mamorekuru.

Iyo yakanyatsogadziriswa silica gel firimu kana girazi faibha firimu yezvinhu zvakaunzwa kunze zvigadzirwa zvinogona kushambadza nucleic acid mamorekuru kusvika pakukwirira kusvika.

AIHWA.	Tsananguro	Filter akaturikidzana	Ruvara rwemhete	Sefa	Cap	Vhoriyamu	Kurongedza / Ctns
PC0001	Spin Column	/	/	/	√	2ml	Bulk	10000
							Bhegi	8000
PC0008	Spin Column	2	Bhuruu	Magirazi egirazi	√	0.8ml	Bulk	12000
							Bhegi	10000
PC0009	Spin Column	4	tsvuku	Magirazi egirazi	√	0.8ml	Bulk	12000
							Bhegi	10000
PC0010	Spin Column	1	/	PTFE firita	√	0.8ml	Bulk	12000
							Bhegi	10000
PC0019	96 tsime rekubvisa ndiro	6	Bhuruu	Silica nhengo	×	96 * 1.0ml	Bhegi	50
PC0027	Spin Column	8	Pepuru	Silica nhengo	√	0.8ml	Bulk	12000
							Bhegi	10000
PC0028	Spin Column	4	Transparent	Silica nhengo	√	0.8ml	Bulk	12000
							Bhegi	10000
PC0033	Spin Column	6	tsvuku	Silica nhengo	√	0.8ml	Bulk	12000
							Bhegi	10000
PC0054	Spin Column	12	Chiedza chitsvuku	Silica nhengo	√	0.8ml	Bulk	12000
							Bhegi	10000
PC0091	O-Mhete	/	/	NC	x	/	Bhegi	5000