Utachiona DNA / RNA Extraltion kit (Column)

Utachiona DNA / RNA Extraltion kit (Column)

Tsananguro Pfupi:


Chigadzirwa Chigadzirwa

Chigadzirwa Mifananidzo

Chigadzirwa Mateki

Iyo DNA yekuchenesa Kit inoshandiswa kune irinyore, inokurumidza uye yakavimbika kuburitsa yemahara kutenderera DNA (CFC-DNA) kubva 1 mL kusvika 4 mL plasma / serum. Iyo yekuchenesa nzira yakavakirwa pane centrifugal column chromatography uye inoshandisa Sigma's proprietary resin kupatsanura matrix. Iyo kit inogona kushandiswa kupatsanura CFC-DNA yemhando dzakasiyana kubva kutsva kana chando plasma / serum sampuli. Uye zvakare, iyo vhoriyamu yeelution inogona kuchinjika kubva ku25 μl kusvika 50 μl. Iyo yakanatswa plasma / serum CFC-DNA ichakwenenzverwa mu eluent kuti iwirirane nechero zasi kwekushandisa, kusanganisira PCR, chaiyo-nguva yakawandisa PCR, methylation inonzwisisika PCR uye Southern blot kuongorora, microarray uye NGS.

Kutenderera DNA yehukuru hwakasiyana kunogona kusarudzika kubva kune plasma uye serum sampuli
Viral uye hutachiona DNA inogona kusarudzika
Inokodzera marudzi ese eplasma uye serum kutanga sampuro saizi (1 mL ~ 4 mL)
Iyo elution vhoriyamu inogona kugadziriswa inoshanduka mukati meiyo 50 μL ~ 100 μL kutarisisa kutenderera DNA
Yemahara kutenderera DNA isina inhibitor inogona kusarudzika
Yakakwira mhando DNA inogona kucheneswa mumaminitsi makumi mana kusvika kumakumi mashanu
Inoenderana neStreck Cell-Yemahara DNA BCT Tubes

Kushanda

PCR
qPCR
Kudhindwa kwekumaodzanyemba
Methylation inonzwisisika PCR
CpG kurongeka
Kudzivirira enzyme kugaya
Kuona hutachiona
Bhakitiriya kuona
microarray
NGS


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